Table 2.

Identified transcription start sites determined by RLM-RACE and associated promoter motifs.

sRNA	−10 Box	−10 Position (bp)	−35 Box	−35 Position (bp)	Start site
17	TGCTTTTAT	−93	TTGCAA	−114	105,761
34	CTTTATAAT	−45	TTGCTA	−69	326,320
47	AATTAAAAT	−65	TGGATA	−89	481,512
60	AGTTACTAT	−43	TTGGTG	−65	844,442
67	AATTATAAT	−36	TTGCAT	−56	998,424
6S	CCTTACTCT	−77	TTATAA	−94	934,943

Using the RLM-RACE sequencing data, the −10 motif and the −35 motif for the σ⁷⁰ promoter were predicted using BPROM. The table lists the predicted −10 nucleotide sequence, the −10 start position upstream from the transcription start site, the −35 nucleotide sequence, and the −35 start position upstream from the transcription start site for the confirmed rickettsial sRNAs. The “Start Site” is the transcription start Site determined by RLM-RACE.