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. 2016 Jun 8;4:48. doi: 10.3389/fbioe.2016.00048

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Copyright © 2016 Panseri, Montesi, Dozio, Savini, Tampieri and Sandri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of scaffold colonization and cell morphology by DAPI and phalloidin staining. After 7 days several cells were seen on MgHA-Gel/Alg scaffold (A) respect to Gel/Alg scaffold (C). MSCs were spread with good morphology and firmly attached to MgHA-Gel/Alg (B) respect to Gel/Alg scaffold (D). Details of cells grown on the inner scaffold surface and on the pore’s edge respectively in (E) and (F). Phalloidin in green stains for actin filaments and DAPI in blue stains for cell nuclei. Scale bars (A–D) 100 μm; (E) 20 μm; (F) 50 μm.