Skip to main content
. 2016 Apr 22;26(6):728–742. doi: 10.1038/cr.2016.48

Figure 3.

Figure 3

Protein analysis of the BF identified decreased expression of ChAT in Chat-Mecp2−/y mice and reduced spontaneous firing of BF cholinergic neurons compared with Chat-IRES-Cre mice. (A) Left: Immunoblotting of ChAT and GAD65 in BF extracts prepared from Chat-IRES-Cre and Chat-Mecp2−/y mice. Each lane was loaded with 40 μg of protein with GAPDH as the loading control. Protein levels of Chat-Mecp2−/y mice were normalized to the corresponding ones of Chat-IRES-Cre mice. Right: Quantification of ChAT and GAD65 proteins in Chat-IRES-Cre and Chat-Mecp2−/y mice. (B) Left: Representative spontaneous firing recordings from BF cholinergic neurons in Chat-IRES-Cre and Chat-Mecp2−/y mice. Right: Quantification of the firing frequency from BF cholinergic neurons in Chat-Mecp2−/y mice normalized by that in Chat-IRES-Cre mice. Error bars are means ± SEM. P-values were calculated by two-sided t-test. *P < 0.05, **P < 0.01, ***P < 0.001.