Table 2.

Thermodynamic parameters of free and entrapped α-amylase.

Temperature (Kelvin)	Free enzyme			Agar entrapped enzyme			Calcium agar entrapped enzyme
	k in × 10−4 (s−1)	E a (kcal/mol)	Half-life (min)	k in × 10−4 (s−1)	E a (kcal/mol)	Half-life (min)	k in × 10−4 (s−1)	E a (kcal/mol)	Half-life (min)
323	1.71	17.60	67.77	1.52	20.04	76.11	1.03	27.49	111.58
328	2.83	17.60	40.75	2.27	20.04	50.91	1.32	27.49	87.64
333	4.47	17.60	25.85	3.29	20.04	35.06	2.02	27.49	57.07
338	6.40	17.60	18.05	5.02	20.04	23.03	3.65	27.49	31.62
343	—	—	—	—	—	—	6.97	27.49	16.59