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. 2016 Jun 7;2:16010. doi: 10.1038/celldisc.2016.10

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Copyright © 2016 SIBS, CAS

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Kdm4b and Kdm5b co-injection greatly facilitates the development of SCNT embryos. (a) Co-injection of Kdm4b+5b greatly improved the preimplantation development rate of SCNT embryos. Both cumulus cells (left panel) and Sertoli cells (right panel) were used as donor cells. (b) Representative images of SCNT embryos injected with Kdm4b+5b mRNA. (c) PCA on SCNT embryos with single mRNA rescued, Kdm4b+5b co-injected and WT embryos at two-cell and four-cell stage. Zygotic genome activation genes were used in two-cell analysis and all expressed genes were used in four-cell-stage analysis. Co-injected SCNT embryos mostly resembled the WT embryos in each stage. (d) The number of differentially expressed genes was reduced by Kdm4b+5b mRNA injection in NT two-cell samples (left panel) and four-cell samples (right panel). (e) Injection of Kdm4b and Kdm4b+5b improved the efficiency of ntESC derivation. The efficiency was calculated on the basis of the total number of blastocysts (left panel) in ntESC derivation or receipt MII oocytes (right panel) in SCNT. (f) Injection of Kdm4b alone or Kdm4b+5b co-injection greatly improved the implantation rates of SCNT embryos and NT-tetraploid-aggregated embryos. The data are represented as the mean±s.d. (n> 3). **P<0.01, ***P<0.001 according to Student’s t-test or the Holm-Sidak test (for analysis of variance). (g) Injection of Kdm4b alone or Kdm4b+5b co-injection greatly improved the birth rates of SCNT embryos and NT-tetraploid-aggregated embryos.