Figure 5. LINC00628 overexpression suppresses GC cells migration and invasion.

A typical Transwell assay was used to examine the impact of LINC00628 on GC cells migration and invasion. 3 × 10⁴ cells in 200 μL serum-free medium were seeded to the top chamber and 500 μL medium with high concentration of serum was added to the bottom. After 12 h, Filters were then submerged in 4% PFA for 15 min and cells on the upper surface were removed by cotton swabs. The cells on the lower surface were stained with hematoxylin-eosin. Ten random fields were selected to determine the average number of cells per view field. For cell invasion assays, the procedure was similar to the cell migration assay, except transwell membranes were precoated with 24 μg/μl Matrigel (BD bioscience, Franklin Lakes, NJ, USA) and the cells were incubated for 8 hr at 37 °C in a 5% CO₂ atmosphere. *P < 0.05.