Figure 6. LINC00628 function as a GC suppressor via suppressing GC tumorigenicity.

(a) LINC00628 knocked down SGC-7901 cells and LINC00628 overexpressed MGC-803 cells were separately plated in 60 mm plates at a density of 1000 cells per plate. For each clone, three independent wells were examined. After 2 weeks of incubation at 37 °C and 5% CO₂, colonies were stained with 0.2% crystal violet and counted. (b,c) Stable SGC-7901 cell with deleted LINC00628 and MGC-803 cell with overexpressed LINC00628 were injected subcutaneously into both hind limbs of the nude mice for 5 weeks. The tumor size was detected every week from the second week from injection. The tumor weight was measured post sacrifice. Results were analyzed by student’s t-test and p < 0.05 was considered significant. *p < 0.05.