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. 2016 Jun 8;6:27435. doi: 10.1038/srep27435

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Copyright © 2016, Macmillan Publishers Limited

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(a) Immunoblotting was employed to detect the expression of LRRN2, CCNA2, HOX11, H3K27me3, EZH2 and LSD1 in LINC00628 knock down SGC-7901 and LINC00628 overexpressed MGC803 cells. The signal of GAPDH was used as loading control. (b) RNA was extracted from nucleus and cytoplasm separately and the expression of LINC00628 was detected by qRT-PCR. (c) Chromatin immunoprecipitation was processed using anti-H3K27me3 antibody in LINC00628 knock down SGC-7901 and LINC00628 overexpressed MGC803 cells. The promoter regions of LRRN2, CCNA2 and HOX11 were detected by qRT-PCR. (d) RNP immunoprecipitation was processed using anti-EZH2 or anti-LSD1 antibody, and RNA was extracted from the pellets. The relative level of LINC00628 was detected by RT-qPCR. The results were analyzed by student’s t-test and p < 0.05 was considered statistically significant. **p < 0.01.