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Relative mRNA expression of ecr (A), broad (B), bhr3 (C), e74a (D), vrille (E) and foxo (F) after 20E and control (CK) treatments of Bactrocera minax pupae. Samples collected in 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h, 72 h and 96 h, after 20E-treatment. Four replications were made. Asterisk shows significant difference (*p < 0.05, **p < 0.01 and ***p < 0.001, t-test).
