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. 2016 Jun 8;6:27376. doi: 10.1038/srep27376

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Copyright © 2016, Macmillan Publishers Limited

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(a) Duplicated set of cells were treated with TNFα and IFNγ (20 ng/ml each), NTP for 5 min as indicated. After 6 h and 24 h after the treatment, each set of cells were harvested and subjected to Western Blot and RT-PCR assay respectively. The data shown are representative of 4 independent experiments. (b) The relative activities of IκB and NF-κB were calculated by measuring the density of each band (total and phosphorylated IκB, p65/RelA) using the Image J analysis program (n = 4), *p < 0.05. TI: TNFα and IFNγ (c) A schematic diagram describing the NTP-mediated p65/RelA regulation.