Figure 6. Combined treatment with NTP and 1% HC effectively reduces the healing duration of AD mice.

(a) A schematic diagram describing the preparation of AD-like mice and the 3 different treatment methods used. (b) The morphological changes in mice after being treated 5 times with each method. The photographs are representative of each group of mice (n = 5), *p < 0.05. (c) The severity of external AD symptoms of the mice were measured by calculating dermatitis score at 0, 2, 4, 6, 9, 12 days after the 1^st application of NTP and 1% HC. *p < 0.05. (d) The results of H&E (top panel), toluidine blue (middle panel), and IHC against EPX (bottom panel). The photographs (X100) are representative of each group of mice (n = 5), scale bar: 100 μm. The total number of the EPX-positive or toluidine blue positive cells were in a picture taken at 400 × magnification were optically counted and averaged for each mouse using 5 randomly selected pictures, *p < 0.05. (e) The results of an IF assay against p65/RelA. DAPI was used for counter-staining the nuclei of cells in the skin tissues. Scale Bar: 50 μm.