Table 3. Property comparison of microbial β-glucosidasesa.
| Species | Enzymes | Optimum |
Km (mM) |
kcat/Km (/s/mM) |
Ki (mM) |
References | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| pH | T (°C) | pNPG | CB | pNPG | CB | pNPG | CB | |||
| Humicola insolens Y1 | HiBgl3A | 5.5 | 60 | 0.90 | 8.44 | 81.6 | 11.1 | 25.0 | – | This work |
| H. insolens Y1 | HiBgl3B | 6.0 | 50 | 1.51 | – | 28.6 | – | 55.2 | – | This work |
| H. insolens Y1 | HiBgl3C | 5.5 | 55 | 0.20 | 6.63 | 1557 | 23.0 | 37.1 | – | This work |
| Aspergillus niger | N188 | 4.8 | 50 | 0.57 | 0.88 | 41 | 36 | 2.7 | – | 30 |
| Aspergillus foetidus | 4.8 | 65 | 0.41 | – | – | – | 8.1 | – | 31 | |
| Aspergillus japonicus | 5.0 | 40 | 0.60 | 0.95 | 432 | 368 | 2.73 | – | 32 | |
| Aspergillus oryzae | 5.0 | 50 | 0.29 | 1.96 | 1270 | 510 | 2.9 | 5 | 33 | |
| Fomitopsis palustris | 5.0 | 50 | 0.12 | 4.8 | 6160 | 21 | 0.35 | – | 34 | |
| Hypocrea jecorina | 5.0 | 50 | 0.09 | 0.75 | 466 | 157 | 0.51 | – | 32 | |
| Myceliophthora thermophila | 5.0 | 40 | 0.39 | 2.64 | 376 | 17 | 0.28 | – | 35 | |
| Trichoderma reesei | 4.5 | 50 | – | 0.54 | – | 41 | – | 0.29 | 36 | |
| Thermoanaerobacterium thermosaccharolyticum DSM 571 | rBGL | 6.4 | 70 | 0.63 | 7.9 | – | 13.3 | 600 | – | 37 |
| Uncultured bacterium | 6.0 | 40 | 0.39 | 20.4 | – | 0.65 | 1000 | – | 38 | |
| Neocallimastix patriciarum | NpaBGS | 6.0 | 40 | – | – | – | – | – | – | 39 |
| Cow rumen metagenome | LAB25g2 | 5.2 | 50 | 0.45 | 4.88 | 0.92 | 0.2 | – | – | 40 |
apNPG was used for the determination of optimal conditions, and pNPG and cellobiose (CB) were used for the determination of kinetics and glucose inhibition.