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. 2016 Jun 8;6:26814. doi: 10.1038/srep26814

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Copyright © 2016, Macmillan Publishers Limited

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CD spectra for (A) full-length GlucY variants and (B) truncated GlucY variants. Secondary structure assignments were computed from the CD data using CDSSTR: (C) change in all variants relative to GlucY; (D) change introduced by C-terminal tyrosine linker relative to native Gluc; (E) change introduced by truncation relative to GlucY; (F) effect of full-length variant mutations relative to GlucY; (G) effect of truncated variant mutations relative to tGY. All samples were measured in CD buffer (see Methods section).