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. 2016 May 25;2016:7917128. doi: 10.1155/2016/7917128

Figure 1.

Figure 1

GTPase assays of various LRRK2 proteins. (a) GTPase assays of the commercially available recombinant LRRK2 proteins. (b) GTPase assays using the immunoprecipitates of myc-tagged LRRK2 proteins transiently expressed in HEK 293T cells. The assay was carried out using a commercial GTPase assay kit. The relative GTPase activity results are shown after a statistical analysis of three independent experiments (top). Each GTPase activity value was divided by the quantity of protein used in the assay measured using GST (a) or myc (b) antibodies. Representative Western blots were shown (bottom). All values are relative to the WT and are expressed as the mean ± SEM. Vec: empty vector, WT: wild type, GS: G2019S, DA: D1994A, RC: R1441C, IT: I2020T, and GR: G2385R. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001.