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. 2016 Jun 8;6:27575. doi: 10.1038/srep27575

Table 1. Effect of different physico-chemical parameters on biosynthesis of gold nanoparticles.

Sample code Physico-Chemical Condition Optimization
Cell free extract concentration (%) Conc. of gold salt (mg/L) pH Time(h) Temp( °C) TEM size(nm) Shape
Effect of cell free extract concentration (%)
 G10.250.7.72.30 10 250 7 72 30 34 ± 20 Mixed
 G50.250.7.72.30 50 250 7 72 30 104 ± 55 Mixed
 G100.250.7.72.30 100 250 7 72 30 197.58 ± 68 Mixed
Effect of concentration of gold salt (mg/L) and Temp(°C)
 G10.250.7.72.30 10 250 7 72 30 34 ± 20 Mixed
 G10.500.7.72.30 10 500 7 72 30 84.77 ± 2.38 Hexagons
 G10.250.7.72.40 10 250 7 72 40 164.90 ± 96.38 Triangles
 G10.500.7.72.40 10 500 7 72 40 337.41 ± 163.09 Triangles
 G10.250.7.72.50 10 250 7 72 50 277.0595 ± 163.18 Triangles
 G10.500.7.72.50 10 500 7 72 50 671.8925 ± 246.95 Triangles
Effect of pH, gold salt concentration and Temp( °C)
 G10.250.5.72.20 10 250 5 72 20 92.7 ± 47.83 Mixed
 G10.250.5.72.30 10 250 5 72 30 61.08 ± 60.00 Mixed
 G10.250.5.72.40 10 250 5 72 40 43 ± 12.37 Pentagons and triangles
 G10.250.5.72.50 10 250 5 72 50 255.65 ± 78.56 Triangles
 G10.500.5.72.20 10 500 5 72 20 42.5 ± 40.5 Mixed
 G10.500.5.72.30 10 500 5 72 30 25.5636 ± 7.48 Pseudospheres
 G10.500.5.72.40 10 500 5 72 40 91.65 ± 62.51 Pentagons and triangles
 G10.500.5.72.50 10 500 5 72 50 475.5 ± 242.40 Triangles
 G10.250.7.72.20 10 250 7 72 20 10.25 ± 2.1 Sheets
 G10.250.7.72.30 10 250 7 72 30 34 ± 20 Mixed
 G10.250.7.72.40 10 250 7 72 40 69.65 ± 58.72 Triangles
 G10.250.7.72.50 10 250 7 72 50 215 ± 89.55 Triangles
 G10.500.7.72.20 10 500 7 72 20 36 ± 5.09 Pseudospheres
 G10.500.7.72.30 10 500 7 72 30 84.77 ± 2.38 Hexagons
 G10.500.7.72.40 10 500 7 72 40 337.41 ± 163.09 Triangles
 G10.500.7.72.50 10 500 7 72 50 671.8925 ± 246.95 Triangles
 G10.250.9.72.20 10 250 9 72 20 32.5 ± 12.68 Mixed
 G10.250.9.72.30 10 250 9 72 30 6.425 ± 2.1 Spheres
 G10.250.9.72.40 10 250 9 72 40 88.5 ± 17.56 Mixed
 G10.250.9.72.50 10 250 9 72 50 356.5 ± 123.59 Triangles
 G10.500.9.72.20 10 500 9 72 20 15 ± 6.88 Mixed
 G10.500.9.72.30 10 500 9 72 30 19 ± 4.08 Spheres
 G10.500.9.72.40 10 500 9 72 40 84.15 ± 14.70 Penta and hexagons
 G10.500.9.72.50 10 500 9 72 50 529.15 ± 262.46 Triangles
Effect of pH
 G10.250.5.72.30 10 250 5 72 30 61.08 ± 60.00 Mixed
 G10.250.5.5.72.30 10 250 5.5 72 30 45.68 ± 23.46 star/hexagonal
 G10.250.6.72.30 10 250 6 72 30 47 ± 28.64 Triangles
 G10.250.6.5.72.30 10 250 6.5 72 30 84.2 ± 61.20 Triangles
 G10.250.7.72.30 10 250 7 72 30 34 ± 20 Mixed
 G10.250.7.5.72.30 10 250 7.5 72 30 46.65 ± 34.84 Triangles
 G10.250.8.72.30 10 250 8 72 30 41.5855 ± 12.27 Triangles
 G10.250.8.5.72.30 10 250 8.5 72 30 59.4374 ± 11.67 penta/hexagons
 G10.250.9.72.30 10 250 9 72 30 6.425 ± 2.1 Spheres
 G10.250.9.5.72.30 10 250 9.5 72 30 4 ± 1.74 Irregular
 G10.250.10.72.30 10 250 10 72 30 4.6 ± 0.98 Irregular
Effect of pH and Time(h)
 G10.250.5.24.30 10 250 5 24 30 11.753 ± 4.26 Pseudospheres
 G10.250.5.48.30 10 250 5 48 30 40.53 ± 39.03 Mixed
 G10.250.5.72.30 10 250 5 72 30 61.08 ± 60.00 Mixed
 G10.250.7.24.30 10 250 7 24 30 57.345 ± 24.93 Mixed
 G10.250.7.48.30 10 250 7 48 30 27 ± 25.01 Mixed
 G10.250.7.72.30 10 250 7 72 30 34 ± 20 Mixed
 G10.250.9.24.30 10 250 9 24 30 5 ± 0.79 Spheres
 G10.250.9.48.30 10 250 9 48 30 5 ± 1.24 Spheres
 G10.250.9.72.30 10 250 9 72 30 6.425 ± 2.1 Spheres

Sample code denotes the physico chemical parameters for synthesis of gold nanoparticles. For example in sample code G10.250.7.72.30, G denotes gold nanoparticles, 10 denotes percent of cell free extract, 250 denotes concentration of gold salt in mg/L, 7 denotes pH, 72 denotes reaction time in hour, and 30 denotes temperature in °C.