Table 1. Effect of different physico-chemical parameters on biosynthesis of gold nanoparticles.
Sample code | Physico-Chemical Condition Optimization | ||||||
---|---|---|---|---|---|---|---|
Cell free extract concentration (%) | Conc. of gold salt (mg/L) | pH | Time(h) | Temp( °C) | TEM size(nm) | Shape | |
Effect of cell free extract concentration (%) | |||||||
G10.250.7.72.30 | 10 | 250 | 7 | 72 | 30 | 34 ± 20 | Mixed |
G50.250.7.72.30 | 50 | 250 | 7 | 72 | 30 | 104 ± 55 | Mixed |
G100.250.7.72.30 | 100 | 250 | 7 | 72 | 30 | 197.58 ± 68 | Mixed |
Effect of concentration of gold salt (mg/L) and Temp(°C) | |||||||
G10.250.7.72.30 | 10 | 250 | 7 | 72 | 30 | 34 ± 20 | Mixed |
G10.500.7.72.30 | 10 | 500 | 7 | 72 | 30 | 84.77 ± 2.38 | Hexagons |
G10.250.7.72.40 | 10 | 250 | 7 | 72 | 40 | 164.90 ± 96.38 | Triangles |
G10.500.7.72.40 | 10 | 500 | 7 | 72 | 40 | 337.41 ± 163.09 | Triangles |
G10.250.7.72.50 | 10 | 250 | 7 | 72 | 50 | 277.0595 ± 163.18 | Triangles |
G10.500.7.72.50 | 10 | 500 | 7 | 72 | 50 | 671.8925 ± 246.95 | Triangles |
Effect of pH, gold salt concentration and Temp( °C) | |||||||
G10.250.5.72.20 | 10 | 250 | 5 | 72 | 20 | 92.7 ± 47.83 | Mixed |
G10.250.5.72.30 | 10 | 250 | 5 | 72 | 30 | 61.08 ± 60.00 | Mixed |
G10.250.5.72.40 | 10 | 250 | 5 | 72 | 40 | 43 ± 12.37 | Pentagons and triangles |
G10.250.5.72.50 | 10 | 250 | 5 | 72 | 50 | 255.65 ± 78.56 | Triangles |
G10.500.5.72.20 | 10 | 500 | 5 | 72 | 20 | 42.5 ± 40.5 | Mixed |
G10.500.5.72.30 | 10 | 500 | 5 | 72 | 30 | 25.5636 ± 7.48 | Pseudospheres |
G10.500.5.72.40 | 10 | 500 | 5 | 72 | 40 | 91.65 ± 62.51 | Pentagons and triangles |
G10.500.5.72.50 | 10 | 500 | 5 | 72 | 50 | 475.5 ± 242.40 | Triangles |
G10.250.7.72.20 | 10 | 250 | 7 | 72 | 20 | 10.25 ± 2.1 | Sheets |
G10.250.7.72.30 | 10 | 250 | 7 | 72 | 30 | 34 ± 20 | Mixed |
G10.250.7.72.40 | 10 | 250 | 7 | 72 | 40 | 69.65 ± 58.72 | Triangles |
G10.250.7.72.50 | 10 | 250 | 7 | 72 | 50 | 215 ± 89.55 | Triangles |
G10.500.7.72.20 | 10 | 500 | 7 | 72 | 20 | 36 ± 5.09 | Pseudospheres |
G10.500.7.72.30 | 10 | 500 | 7 | 72 | 30 | 84.77 ± 2.38 | Hexagons |
G10.500.7.72.40 | 10 | 500 | 7 | 72 | 40 | 337.41 ± 163.09 | Triangles |
G10.500.7.72.50 | 10 | 500 | 7 | 72 | 50 | 671.8925 ± 246.95 | Triangles |
G10.250.9.72.20 | 10 | 250 | 9 | 72 | 20 | 32.5 ± 12.68 | Mixed |
G10.250.9.72.30 | 10 | 250 | 9 | 72 | 30 | 6.425 ± 2.1 | Spheres |
G10.250.9.72.40 | 10 | 250 | 9 | 72 | 40 | 88.5 ± 17.56 | Mixed |
G10.250.9.72.50 | 10 | 250 | 9 | 72 | 50 | 356.5 ± 123.59 | Triangles |
G10.500.9.72.20 | 10 | 500 | 9 | 72 | 20 | 15 ± 6.88 | Mixed |
G10.500.9.72.30 | 10 | 500 | 9 | 72 | 30 | 19 ± 4.08 | Spheres |
G10.500.9.72.40 | 10 | 500 | 9 | 72 | 40 | 84.15 ± 14.70 | Penta and hexagons |
G10.500.9.72.50 | 10 | 500 | 9 | 72 | 50 | 529.15 ± 262.46 | Triangles |
Effect of pH | |||||||
G10.250.5.72.30 | 10 | 250 | 5 | 72 | 30 | 61.08 ± 60.00 | Mixed |
G10.250.5.5.72.30 | 10 | 250 | 5.5 | 72 | 30 | 45.68 ± 23.46 | star/hexagonal |
G10.250.6.72.30 | 10 | 250 | 6 | 72 | 30 | 47 ± 28.64 | Triangles |
G10.250.6.5.72.30 | 10 | 250 | 6.5 | 72 | 30 | 84.2 ± 61.20 | Triangles |
G10.250.7.72.30 | 10 | 250 | 7 | 72 | 30 | 34 ± 20 | Mixed |
G10.250.7.5.72.30 | 10 | 250 | 7.5 | 72 | 30 | 46.65 ± 34.84 | Triangles |
G10.250.8.72.30 | 10 | 250 | 8 | 72 | 30 | 41.5855 ± 12.27 | Triangles |
G10.250.8.5.72.30 | 10 | 250 | 8.5 | 72 | 30 | 59.4374 ± 11.67 | penta/hexagons |
G10.250.9.72.30 | 10 | 250 | 9 | 72 | 30 | 6.425 ± 2.1 | Spheres |
G10.250.9.5.72.30 | 10 | 250 | 9.5 | 72 | 30 | 4 ± 1.74 | Irregular |
G10.250.10.72.30 | 10 | 250 | 10 | 72 | 30 | 4.6 ± 0.98 | Irregular |
Effect of pH and Time(h) | |||||||
G10.250.5.24.30 | 10 | 250 | 5 | 24 | 30 | 11.753 ± 4.26 | Pseudospheres |
G10.250.5.48.30 | 10 | 250 | 5 | 48 | 30 | 40.53 ± 39.03 | Mixed |
G10.250.5.72.30 | 10 | 250 | 5 | 72 | 30 | 61.08 ± 60.00 | Mixed |
G10.250.7.24.30 | 10 | 250 | 7 | 24 | 30 | 57.345 ± 24.93 | Mixed |
G10.250.7.48.30 | 10 | 250 | 7 | 48 | 30 | 27 ± 25.01 | Mixed |
G10.250.7.72.30 | 10 | 250 | 7 | 72 | 30 | 34 ± 20 | Mixed |
G10.250.9.24.30 | 10 | 250 | 9 | 24 | 30 | 5 ± 0.79 | Spheres |
G10.250.9.48.30 | 10 | 250 | 9 | 48 | 30 | 5 ± 1.24 | Spheres |
G10.250.9.72.30 | 10 | 250 | 9 | 72 | 30 | 6.425 ± 2.1 | Spheres |
Sample code denotes the physico chemical parameters for synthesis of gold nanoparticles. For example in sample code G10.250.7.72.30, G denotes gold nanoparticles, 10 denotes percent of cell free extract, 250 denotes concentration of gold salt in mg/L, 7 denotes pH, 72 denotes reaction time in hour, and 30 denotes temperature in °C.