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. 2016 Jun 8;6:27636. doi: 10.1038/srep27636

Figure 1. Glucose uptake and utilisation mutants have attenuated growth in minimal medium.

Figure 1

P. aeruginosa PAO1 glucose uptake operon (A). Location of glucose uptake and utilisation genes in P. aeruginosa carbon metabolism pathway, genes selected for deletion underlined (B). Growth of mutant strains ΔoprB, ΔgltK, ΔgtrS and Δglk was assessed by optical density after inoculation of overnight cultures 1:100 in LB (C), M9 with 50 mM glucose (D), 50 mM fructose (E) or 50 mM succinate (F). Points represent the mean +/− SD of n = 3 replicates. *p < 0.01, ***p < 0.001 between PAO1 and other mutants at different glucose concentrations, #p < 0.05, ###p < 0.001 between ΔoprB and ΔgtrS by two way ANOVA with post test.