Figure 3. Acute hyperglycaemia leads to increased bacterial load after wild type PAO1 infection.

Mice were injected with 2 doses of 10 mg/dose streptozocin intraperitoneally (STZ, closed squares) or untreated (open squares). Glucose was measured in blood (A) or BAL (B) prior to infection. Log phase growth PAO1 was administered intranasally at a concentration of 10⁶ CFU/mouse. Bacterial burden was measured in BAL (C). Weight change was measured (D) and percentage airway neutrophils (E) and IL-6 were measured in the BAL (F). Points represent individual mice (A–C,E,F) or mean +/− SD of n = 5 animals. Lines represent mean values. *p < 0.05, **p < 0.01, ***p < 0.001, calculated using multiple t tests with weighted corrections.