Figure 4. Hyperglycaemia increases acute bacterial load in mice infected with WT but not knockout strains.

Mice were injected with 2 doses of 10 mg/dose Streptozocin (STZ) intraperitoneally, and mice were infected intranasally with 10⁶ CFU/mouse of either WT PAO1 or mutant strains and sacrificed after 24 hours. BAL CFU (A), fold change in BAL CFU between STZ and control animals (B), BAL neutrophilia (C) and IL-6 (D) were assessed at 24 hours after infection. Points represent individual mice, lines represent mean values, bars represent mean +/− SEM of n = 5 animals. *p < 0.05, calculated using multiple t tests with weighted corrections.