Figure 1. MIF expression and distribution in acute injured liver induced by CCl₄.

After CCl₄ administration at 24 hours, 48 hours and 72 hours, mRNA (a) and protein (b) expression of MIF in mice livers were measured by real-time RT-PCR and Western blot, respectively. (c) The quantitative assay of Western blot was executed by Odyssey® software. The representative images of immunohistochemical staining (d) and HE staining (f) were shown to track MIF (brown) in liver tissue of normal or CCl₄–treated mice. (e) The mean optical density in immunohistochemical staining images of MIF was measured by Image-Pro Plus software. Scale bars: 200 μm. Data are presented as the means ± SEM. *P < 0.05 vs control group (n = 6, per group).