Figure 6. MIF inhibitor (ISO-1) decreased expression of MCP-1 and phosphorylation of p38 in CCl₄-treated liver.

At 15 minutes before CCl₄ administration, mice received an injection of ISO-1 (35 mg/kg body weight), 48 hours later, liver tissue were collected. (a) mRNA expression of MCP-1 were measured by real-time RT-PCR. (c) Protein level of total p38 and phosphor-p38 were evaluated by Western blot analysis. (b) The quantitative assay of Western blot was executed by Odyssey® software. Data are presented as the means ± SEM. All results were confirmed in three independent experiments at least. *P < 0.05 vs control group (n = 6, per group).