Figure 2.

(a) Analysis of growth. Growth pattern of LdHAT4-KO cells was compared with those of wild type Ld1S and Ld1S-neo/hyg control lines. (b) Analysis of cell survival. Percent LdHAT4-KO survivors was compared with percent survivors in control lines, every 24 hrs over a period of seven days. For growth and cell survival analyses, three biological replicates were initiated and carried out in parallel, and mean values are presented, with error bars indicating standard deviation. Student’s t-test (two-tailed) was applied to analyze the data. P values obtained: ns₌non-significant, *p < 0.05, **p < 0.005, ***p < 0.0005. (c) Analysis of DNA-associated and soluble protein fractions. Left panels: S1 to S4 lysate fractions isolated from logarithmically growing cells were resolved by SDS-PAGE (5 × 10⁶ cell equivalents per cell type) and analyzed by western blot with anti-unmodified H4 antibodies (1:10000 dilution¹³) and anti-H4acetylK4 antibodies (1:1000 dilution¹³). S1, S2: soluble fractions. S3, S4: DNA-associated fractions. Right panel: ratio of DNA associated H4 (modified + unmodified) : soluble H4 (modified + unmodified) in Ld1S-neo/hyg and HAT4-KO cells, as determined by quantification using Image J.