Figure 5. The central protuberance assembly factors BXDC1 and RRS1 are required for nucleolar structure integrity.

(a) Cells expressing FBL fused to the GFP were treated for 3 days with an siRNA targeting transcripts encoding the indicated protein. Two independent siRNAs (#1 and #2) were used in each case. Cells treated with a non-targeting (SCR) siRNA control are shown for reference. (b) For each depletion, the nucleolar disruption index (iNo) was calculated (see Fig. 1 and Methods section).