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. 2016 Jun 6;7:11810. doi: 10.1038/ncomms11810

Figure 1. FD curves and their derivatives of torsionally constrained and torsionally relaxed DNA.

Figure 1

(a) Average FD curves of torsionally constrained DNA (grey) and torsionally relaxed DNA (black) in 25 mM NaCl. Inset highlights the construct design for torsionally constrained DNA and torsionally relaxed DNA. In both cases, each end of the DNA molecule is ligated to an end-cap labelled with biotin moieties (orange). The DNA is only torsionally constrained if ≥2 biotins on each end of the molecule are bound to streptavidin-coated optically trapped beads. (b) First derivative of the FD curves displayed in a. (c) second derivative of the FD curves displayed in a, obtained using a Savitzky-Golay (20 point) smoothing filter. Dashed lines indicate where the four peaks (T1–T4) identified in c lie in the FD curves.