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. 2016 May 10;4:1422. Originally published 2015 Dec 10. [Version 2] doi: 10.12688/f1000research.7507.2

Figure 3. One Codex classification of ITS reads.

Figure 3.

P. paxilli is the most frequently found contaminant in Cannabis flowers. P. citrinum is not in the One Codex database at this time. One Codex utilizes a fast k-mer based approach for whole genome shotgun classification and can be influenced by read trimming and database content. The reads provided to MG-RAST were trimmed and FLASH’d (paired end reads merged when overlapping) prior to classification. K-mer based approaches can significantly differ from longer word size methods and this underscores the importance of confirmatory PCR in microbiome analysis.