Skip to main content
. 2016 Jun 8;6:27558. doi: 10.1038/srep27558

Figure 3. High glucose induces activation of canonical Wnt signaling.

Figure 3

(A–F) HepG2 and SK-HEP-1 cells were cultured in NG, HG and HG + CytoB for 16 hr. Whole cell lysates were subjected to western blotting and levels of β–catenin, pJNK and JNK proteins were detected. (B) HepG2 cells were serum and glucose starved for 2 hr and then cultured in HG for indicated time intervals. Whole cell lysates were prepared and β-catenin protein level was detected by western blotting. (C) Cytosolic and nuclear extracts were prepared from HepG2 cells cultured in NG, HG and HG + CytoB for 16 hr and processed for immunodetection of β–catenin levels. Cropped blots are used in the main figure and full length blots are included in Supplementary Figure 8. (D) HepG2 cells were cultured in NG, HG and HG + CytoB for 16 hr and cells were processed for immunofluorosence based confocal imaging of β–catenin protein. Bars represent 10 μm. (E) β–catenin transcription activity was determined by TCF reporter assay in HepG2 cells cultured in NG, HG and HG + CytoB for 16 hr. The luciferase intensities were normalized with Renilla intensities and data is represented as ratio of TOP/FOP. Bar graph represents mean ± SE of three independent experiments (*P < 0.05, **P < 0.001, ***P < 0.0001).