Table 1.
Biogas fermenter May 2013 sample | #Reads used for assembly | Ray assembly | IDBA-UD assembly | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
#Contigs total | #Contigs >1000 bp | N50 for contigs >1000 bp | Mb in contigs >1000 bp | Mb total | #Contigs total | #Contigs >1000 bp | N50 for contigs >1000 bp | Mb in contigs >1000 bp | Mb total | ||
DNA isolation 1 | 159,458,382 | 1,201,371 | 57,009 | 7183 | 209.7 | 486.0 | 556,160 | 116,123 | 5587 | 404.7 | 613.8 |
DNA isolation 2 | 421,986,642 | 2,003,618 | 94,702 | 11,536 | 425.9 | 876.6 | 947,772 | 200,495 | 5976 | 724.0 | 1069.9 |
DNA isolation 1 + 2 | 581,445,024 | 2,319,807 | 112,571 | 10,871 | 512 | 1,035.8 | 1,142,608 | 236,489 | 5823 | 843.0 | 1255.4a |
DNA isolation 2 + additional sequencing of isolation 2 | 737,631,618 | 2,593,366 | 123,435 | 12,418 | 581.3 | 1161.7b | |||||
DNA isolation 1 + 2 + additional sequencing of isolation 2 | 897,090,000 | 2,826,937 | 140,535 | 11,784 | 653.73 | 1292.2 |
a Assembly uploaded to IMG/MER and used for the phylogenetic and comparative analyses
b Assembly used for metagenomic binning