. 2016 Jun 7;9:326. doi: 10.1186/s13071-016-1613-3

Table 1.

Nucleotide sequences of polymerase chain reaction primers and conditions used for amplification of Bartonella spp. and host genes from land leeches

Target gene	PCR	Name of the PCR primer used	Sequence (5′–3′)	Annealing temperature	Amplicon size (bp)	Reference
Bartonella spp. ITS	1^st	QHVE1	TTCAGATGATGATCCCAAGC	58	735	[21]
	1^st	QHVE4	AACATGTCTGAATATATCTTC	58	735
	2^nd	QHVE12	CCGGAGGGCTTGTAGCTCAG	58	484
	2^nd	QHVE14	CACAATTTCAATAGAAC	58	484
Anaplasma spp.16S rRNA	1^st	AE1-F	AAGCTTAACACATGCAAGTCGAA	56	1,406	[21]
Anaplasma spp.16S rRNA	1^st	AE1-R	AGTCACTGACCCAACCTTAAATG	56	1,406
Anaplasma phagocytophilum 16S rRNA	2^nd	EE3	GTCGAACGGATTATTTTTATAGCTTGC	56	926
Anaplasma phagocytophilum 16S rRNA	2^nd	EE4	CCCTTCCGTTAAGAAGGATCTAATCTCC	56	926
Anaplasma bovis 16S rRNA	2^nd	ABkf	TAGCTTGCTATGGGGACAA	59	547
Anaplasma bovis 16S rRNA	2^nd	ABlr	TCTCCCGCACTCCAGTCTG	59	547
Ehrlichia spp. 16S rRNA	1^st	ECC	AGAACGAACGCTGGCGGCAAGC	56	450	[22]
Ehrlichia spp. 16S rRNA	1^st	ECB	CGTATTACCGCGGCGCTGGCA	56	450
Ehrlichia chaffeensis 16S rRNA	2^nd	HE3	TATAGGTACCGTACTTATCTTCCCTAT	56	390
Ehrlichia chaffeensis 16S rRNA	2^nd	HE1	CAATTGCTTATAACCCTTTTGGTTATAAAT	56	390
Ehrlichia canis 16S rRNA	2^nd	HE3	TATAGGTACCGTACTTATCTTCCCTAT	56	365
Ehrlichia canis 16S rRNA	2^nd	ECAN5	CAATTATTTATAGCCTCTGGCTATAGGC	56	365
Borrelia burgdoferi 16S rRNA	1^st	B1	CAGTGCGTCTTAAGCATGC	59	1,427	[21]
	1^st	B8	CCTTAAATACCTTCTCCC	59	1,427
	2^nd	B3	GCAGCTAAGAATCTTCCGCAATGG	60	714
	2^nd	B6	CAACCATGCAGCACTGTATAT	60	714
Rickettsia sp. gltA	1^st	RpCS. 877p	GGGGACCTGCTCACGGCGG	54	382	[23]
	1^st	RpCS. 1258n	ATTGCAAAAAGTACAGTGAA	54	382
	2^nd	RpCS. 896p	GGCTAATGAAGCAGTGATAA	58	338
	2^nd	RpCS. 1233n	GCGACGGTATACCCATAG	58	338
Wolbachia 16S rRNA		16S-F	TTGTAGCCTGCTATGGTATAACT	56	937	[24]
Wolbachia 16S rRNA		16S-R	GAATAGGAGTTTTCATGT	56	937	[24]
mt DNA cytochrome b gene		L14841	AAAAAGCTTCCATCCAACATCTCAGCATGATG	50	450	[25]
mt DNA cytochrome b gene		H15149	AAACTGCAGCCCCTCAGAATGATATTTGTCCTCA	50	450	[25]