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. 2016 Jun 8;6:27615. doi: 10.1038/srep27615

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PBMCs were pretreated with PGD₂, PGE₂, or combination of PGD₂ and PGE₂ and then incubated with autologous granulocytes. (a) Representative FACS profiles showing the effect of PGs on the percentage of annexin V+ eosinophils. (b) Statistical line graphs showing the percent response of annexin V+ eosinophils to different concentrations of PGs relative to that with vehicle only. Box and whisker plots show the median and the minimum to maximum percentiles. *P < 0.01; **P < 0.001 by repeated measure ANOVA with the Friedman’s test.