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. 2016 Jun 7;12:31. doi: 10.1186/s13007-016-0131-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Root and nodule growth dynamic obtained by digital imaging analysis. Images of pea plant (kayanne genotype) grown in RhizoTube without soil mineral nitrogen in experiment 3 were realized using RhizoCab at resolution of 600 ppi and RVB light. Examples of nodulated roots images (with a focus in the yellow colored square) taken at 309, 464 and 597 degree-days are shown in a, b and c respectively. Image segmentation allows to obtain the pixels numbers for roots and nodules. These values are then converted in biomass (grams) using a calibration curves (data not shown). As such, nodule biomass (open circles) and root biomass (closed circles) can be dynamically estimated during the growth cycle (d)