Figure 2. MicroRNA-21 (miR-21) directly targeting programmed cell death 4 (PDCD4). A: combined sequences of PDCD4 mRNA and microRNA-21 in 3′-UTR binding site; B, Dual-luciferase reporter analysis results: the activity of luciferase showed no significant difference in the group transfected with carriers of miR-21 and mutant (MT) PDCD4 mRNA (P>0.05), while the activity of luciferase was significantly decreased in the group transfected with carriers of miR-21 and wild type (WT) PDCD4 mRNA expressions (P<0.01); *P<0.05, pcDNA3.1-miR-21 group (transfected) compared to pcDNA3.1 group (negative control) (Student t-test).
