Figure 5. Programmed cell death 4 (PDCD4), c-Jun and p-c-Jun expressions after transfection. A, Western blot analysis of each group; B, comparison of PDCD4, c-Jun, and p-c-Jun expressions in each group. The expression of PDCD4 was decreased in both PDCD4 siRNA group and miR-21 mimics group, but the expression of c-Jun was elevated in both groups. The inhibition of miR-21 led to an increase of PDCD4 expression and decrease of c-Jun expression, while no differences in p-c-Jun expressions were detected among the five groups. *P<0.05, compared with blank control (MOCK) and negative control (NC) groups; ^#P<0.05 compared with miR-21 inhibitor group. Comparisons among multiple groups were conducted using one-way ANOVA and pairwise comparisons were conducted using t-test.