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. Author manuscript; available in PMC: 2016 Sep 1.

Published in final edited form as: Anal Bioanal Chem. 2015 Jul 10;407(23):7003–7012. doi: 10.1007/s00216-015-8865-1

Fig. 6 — Stimulation optimization. RAW 264.7 macrophage cells were stimulated with 2 ng/mL PMA for 12 h and then incubated with 6 μL of 5 mM MitoHE for 10 min. a Fluorescence image of stimulated cells and b fluorescence image of native/control cells after stimulation protocol. c Electropherogram for (I) stimulated RAW 264.7 in comparison to (II) standard containing MitoE and 2-OH-MitoE. Detection point = 2.5 cm from T intersection

Fig. 6 — Stimulation optimization. RAW 264.7 macrophage cells were stimulated with 2 ng/mL PMA for 12 h and then incubated with 6 μL of 5 mM MitoHE for 10 min. a Fluorescence image of stimulated cells and b fluorescence image of native/control cells after stimulation protocol. c Electropherogram for (I) stimulated RAW 264.7 in comparison to (II) standard containing MitoE and 2-OH-MitoE. Detection point = 2.5 cm from T intersection