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. Author manuscript; available in PMC: 2016 Jun 8.
Published in final edited form as: Biochem J. 2012 Jun 15;444(3):561–571. doi: 10.1042/BJ20120294

Figure 6. Regulation of mitochondrial function by metabolic substrate supply.

Figure 6

MCF7 cells were seeded in specialized microplates and cultured for 24h. Cells were then switched to unbuffered DMEM containing glucose only (5.5.6 mM), pyruvate only (1 mM), glucose and pyruvate (complete media), or complete media with CHC (500 µM) 1 h prior to measuring mitochondrial function. Basal and maximal OCR (A) and the reserve capacity (B) were measured using sequential ingjection of oligomycin, FCCP, and Antimycin A. OCRs were normalized to total protein/well after completion of assay. Values represent means ± SEM, n=3–5. * p < 0.05 compared to complete media.

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