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(a) Thermostability of the Ebeacon system at different temperatures from 20 °C to 50 °C. As the reaction temperature increased from 20 °C to 50 °C, the background remained relatively stable for 1 h. Even at 50 °C, the background signal enhancement was less than 2-fold, compared with the initial signal. (b) Selectivity toward single-base substitution (mC, mG, mT), insertion (iC, iG, iT, iA) and deletion (dA).
