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. 2015 Oct 6;7(43):24069–24074. doi: 10.1021/acsami.5b06987

Figure 1.

Figure 1

Schematic illustration of self-assembled DNA immuno-NFs for immunostimulation. The linear DNA templates composed of CpG complements and linkers and FITC-labeled primers were first annealed and ligated to form a circular template. RCR was performed by using Φ29 DNA polymerase to generate a large amount of elongated nonnicked concatemer DNA with each unit complementary to the template. NFs were then self-assembled via liquid crystallization. After being uptaken by macrophages, these NFs could be recognized by TLR9, followed by the secretion of cytokines.