Table 1.
Comparison of the levels of inflammatory cytokines and chemokines in the BAL fluid of C57BL/6 female and male mice 3 months after repeated exposure with CNC
| Cytokines | Control (pg/ml) | Females (fold of control) | Males (fold of control) |
|---|---|---|---|
| IL-1α | 1.11 ± 0.05 | 1.78 ± 0.08* | 1.87 ± 0.10* |
| IL-1β | 24.75 ± 0.70 | 2.21 ± 0.12*# | 1.68 ± 0.08* |
| IL-2 | 1.95 ± 0.11 | 1.73 ± 0.12* | 1.69 ± 0.19* |
| IL-4 | 2.68 ± 0.03 | 1.34 ± 0.04* | 1.29 ± 0.02* |
| IL-5 | 1.77 ± 0.10 | 1.87 ± 0.11*# | 0.85 ± 0.09 |
| IL-6 | 3.92 ± 0.17 | 1.29 ± 0.08* | 1.08 ± 0.05 |
| IL-10 | 6.83 ± 0.27 | 1.53 ± 0.08*# | 1.28 ± 0.12* |
| IL-12(p40) | 110.30 ± 7.11α | 3.80 ± 0.26* | 3.20 ± 0.27* |
| 66.76 ± 1.99β | |||
| IL-12(p70) | 24.95 ± 0.80 | 1.36 ± 0.07*# | 1.17 ± 0.04 |
| IL-13 | 113.39 ± 3.09 | 1.27 ± 0.04* | 1.16 ± 0.05 |
| IL-17A | 4.20 ± 0.20 | 1.51 ± 0.06*# | 0.99 ± 0.08 |
| Eotaxin | 468.96 ± 32.56 | 1.15 ± 0.07 | 1.32 ± 0.07* |
| G-CSF | 6.77 ± 0.19 | 1.32 ± 0.05* | 1.18 ± 0.08* |
| KC | 7.73 ± 0.21 | 4.16 ± 0.56*# | 2.33 ± 0.18* |
| MCP-1 | 29.30 ± 0.72 | 8.15 ± 1.7*# | 2.35 ± 0.26* |
| MIP-1α | 5.81 ± 0.16 | 13.43 ± 1.63*# | 6.51 ± 0.64* |
| MIP-1β | 3.50 ± 0.18 | 2.17 ± 0.12*# | 1.42 ± 0.09* |
| RANTES | 2.37 ± 0.22 | 1.87 ± 0.15* | 1.90 ± 0.28* |
The control values otherwise noted correspond to the mean absolute values (pg/ml) from PBS-exposed male mice. The PBS-exposed control female and male mice were not significantly different, except for IL-12(p40). Level of IL-12(p40) for control femalesα mice (110.30 ± 7.11 pg/ml) was significantly different as compared to control malesβ mice (66.76 ± 1.99 pg/ml). For comparison, cytokines data presented as folds of control for female and male mice exposed to CNC. Mean ± SEM (n = 10 mice/group). *p < 0.05, vs control PBS-exposed mice, # p < 0.05, vs male mice exposed to CNC