Table 3.

Comparison of different solubilization agents for recovery of hGH from IBs

Solubilization agent	Refolding yield (%)	Overall recovery (%)	References
50 mM Tris, pH 8.5, 8 M urea	50	21	[14, 18]
50 mM Tris, pH 12, 2 M urea	86	42	[17, this study]
50 mM Tris, pH 8.5, 6 M n-propanol, 2 M urea	76	40	[14]
50 mM Tris, pH 8.5, 30 % TFE, 3 M urea	87	45	This study