Table 5.
Differentially accumulated proteins with similar functionsin Qi319-96 and Qi319 leaves under both + P and − P conditions
| Matched protein namea | gi Numberb | Spot no.c | Patternd | Qi319-96– P versus Qi319-96 + Pe | Qi319– P versus Qi319 + Pf |
|---|---|---|---|---|---|
| Qi319-96 + P/Qi319 + P | |||||
| Metabolism | |||||
| Chlorophyll A-B binding protein | gi|242088861 | M1 | Increase | 1:1.04 | 1.07:1 |
| Chlorophyll A-B binding protein | gi|194700378 | M2 | Increase | 1:1.70 | 1: 5.05 |
| Chain A, Pyruvate phosphate dikinase | gi|62738111 | M3 | Increase | 1.62:1 | 1.78:1 |
| malate dehydrogenase | gi|226498728 | M4 | Increase | 1:1.74 | 1:2.03 |
| Coproporphyrinogen III oxidase | gi|308081534 | M5 | Increase | 6.93:1 | 1:60.35 |
| Triosephosphate isomerase | gi|194702698 | M9 | Increase | 1.75:1 | 1:2.96 |
| hydroxypyruvate reductase | gi|194697898 | M10 | Decrease | 1.30:1 | 1.07:1 |
| thylakoid lumenal 19 KDa protein | gi|226491484 | M11 | Decrease | 4.89:1 | 2.72:1 |
| sedoheptulose bisphosphatase1 | gi|226506366 | M12 | Decrease | 1:1.12 | 1:1.72 |
| Cyanobacterial and plastid NDH-1 subunit M | gi|194701566 | M13 | Decrease | 1:7.25 | 1:2.25 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|144583482 | M19 | Decrease | 1:19.87 | 5.91:1 |
| Ribulose bisphosphate carboxylase large chain | gi|132061 | M20 | Decrease | 1:6.24 | 1:3.99 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|11467200 | M21 | Decrease | 1:1.11 | 1:3.09 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|260677427 | M22 | Decrease | 1.30:1 | 1.08:1 |
| chlorophyll a-b binding protein 8 | gi|195613254 | M23 | Decrease | 1:3.41 | 1:2.12 |
| oxygen-evolving enhancer protein 1 | gi|195619530 | M26 | Decrease | 1:2.45 | 1:4.89 |
| NADH dehydrogenase subunit I | gi|11467259 | M28 | Decrease | 1:8.84 | 1:1.71 |
| Energy | |||||
| GlyoxalaseI | gi|212274373 | M16 | Decrease | 1:1.27 | 8.09:1 |
| glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic | gi|162461856 | M17 | Decrease | 1:1.49 | 1:1.39 |
| GADPH (383 AA) | gi|22240 | M29 | Decrease | 7.99:1 | 2.09:1 |
| Protein fate | |||||
| peptidyl-prolyl cis-trans isomerase | gi|226491656 | M6 | Increase | 1:18.53 | 1:35.85 |
| chaperonin | gi|195623400 | M14 | Decrease | 1:1.19 | 2.20:1 |
| CPN10 | gi|194688414 | M15 | Decrease | 1:1.50 | 3.19:1 |
| Pepsin-like aspartate proteases | gi|168041407 | M24 | Decrease | 1:1.39 | 1:16.85 |
| peptide methionine sulfoxide reductase | gi|226532399 | M27 | Decrease | 4.14:1 | 3.16:1 |
| Protein synthesis | |||||
| ribonucleoprotein | gi|22942613 | M18 | Decrease | 2.47:1 | 1:17.3 |
| Transcription/cellular communication/signal transduction | |||||
| glycine-rich RNA binding protein 2 | gi|195609654 | M7 | Increase | 1:10.72 | 1:15.2 |
| Unknown | |||||
| hypothetical protein SORBIDRAFT_09g001130 | gi|242086601 | M8 | Increase | 4.21:1 | 1.17:1 |
| LOC542632 | gi|162462462 | M25 | Decrease | 10.09:1 | 1.24:1 |
| Qi319-96 – P/Qi319 – P | |||||
| Metabolism | |||||
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|144583490 | N3 | Increase | 1:7.29 | 1:3.06 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|27448357 | N4 | Increase | 1:1.35 | 1:2.99 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|290086785 | N5 | Increase | 1:1.89 | 2.41:1 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|135991663 | N6 | Increase | 1:6.23 | 1:1.87 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|3560858 | N7 | Increase | 1.86:1 | 1:12.11 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|224382434 | N8 | Increase | 4.21:1 | 6.33:1 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|11467200 | N9 | Increase | 1:16.96 | 1:19.00 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|144583486 | N11 | Increase | 1:4.14 | 1:3.37 |
| Ribulose bisphosphate carboxylase large chain | gi|261279200 | N12 | Increase | 1:4.78 | 1:6.44 |
| ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|164565217 | N14 | Increase | 1:6.53 | 1:3.59 |
| chlorophyll a-b binding protein 8 | gi|226496924 | N15 | Increase | 1:7.07 | 9.89:1 |
| Chlorophyll a-b binding protein6A | gi|223973225 | N16 | Increase | 1:1.33 | 1.33:1 |
| chlorophyll a-b binding protein 8 | gi|195613254 | N17 | Increase | 3.79:1 | 1:10.14 |
| UDP-sulfoquinovose synthase | gi|238014584 | N18 | Increase | 4.69:1 | 3.63:1 |
| NDH-dependent cyclic electron flow 1 NAD-dependent epimerase | gi|194704742 | N19 | Increase | 1:1.33 | 1:2.93 |
| sucrose-phosphatase 1 | gi|194699500 | N21 | Increase | 1:1.79 | 1:6.51 |
| NADP-dependent malic enzyme,chloroplastic | gi|162459265 | N32 | Increase | 1.27:1 | 1:5.15 |
| NADP-dependent malic enzyme, chloroplastic precursor | gi|162459265 | N35 | Qi319-96 only | 1:1.59 | 0:1 |
| NADP-dependent malic enzyme, chloroplastic | gi|162459265 | N36 | Increase | 1.05:1 | 1:8.35 |
| pyruvate orthophosphate dikinase | gi|285013667 | N38 | Increase | 2.24:1 | 4.35:1 |
| pyruvateorthophosphate dikinase | gi|168586 | N39 | Increase | 1:1.66 | 1:7.35 |
| pyruvate orthophosphate dikinase | gi|219819651 | N41 | Qi319-96 only | 1:2.26 | 0:1 |
| delta-aminolevulinic acid dehydratase | gi|226496321 | N43 | Increase | 1:2.07 | 1:2.96 |
| transferase, transferring glycosyl groups, putative | gi|255562878 | N44 | Increase | 1:4.52 | 1:6.58 |
| glucose-6-phosphate isomerase | gi|226530882 | N51 | Increase | 1:1.31 | 1:1.91 |
| Low PSII Accumulation 3 | gi|242060200 | N54 | Increase | 2.07:1 | 2.59:1 |
| subunit NDH-M of NAD(P)H:plastoquinone dehydrogenase | gi|226497418 | N55 | Increase | 1:1.85 | 1.08:1 |
| carbonate dehydratase | gi|293332983 | N69 | Decrease | 1:2.58 | 1:3.07 |
| thylakoid lumenal 19 kDa protein | gi|226491484 | N71 | Decrease | 1:1.83 | 3.33:1 |
| Energy | |||||
| NADP+-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase B | gi|194688752 | N1 | Increase | 1:1.72 | 1:1.98 |
| NADP+-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase B | gi|194700892 | N2 | Increase | 2.04:1 | 1:1.68 |
| 6-phosphogluconate dehydrogenase family protein | gi|162463282 | N10 | Increase | 1.99:1 | 1:1.50 |
| alcohol dehydrogenase 2 | gi|195613268 | N20 | Increase | 1:1.02 | 1:1.68 |
| phosphoglucomutase, cytoplasmic 1 | gi|162463106 | N23 | Increase | 2.21:1 | 1:3.65 |
| fructose-bisphosphate aldolase | gi|195622374 | N26 | Increase | 8.56:1 | 1:4.94 |
| fructose-bisphosphate aldolase | gi|223975775 | N27 | Increase | 1:4.39 | 1:3.22 |
| fructose-bisphosphate aldolase | gi|194690156 | N28 | Increase | 2.90:1 | 2.18:1 |
| fructose-bisphosphate aldolase | gi|195634659 | N29 | Increase | 1.41:1 | 1.38:1 |
| aconitase | gi|238014964 | N33 | Increase | 2.05:1 | 1:4.53 |
| Glyceraldehyde-3-phosphate dehydrogenase B, chloroplast | gi|108705994 | N34 | Increase | 1:5.96 | 1:2.29 |
| phosphoglucomutase, cytoplasmic 1 | gi|162463106 | N37 | Increase | 1.26:1 | 1:9.30 |
| phosphoglycerate mutase | gi|293336560 | N45 | Increase | 1:1.40 | 1:3.19 |
| pyruvate dehydrogenase E1 component subunit beta | gi|195621752 | N48 | Increase | 4.55:1 | 1:2.99 |
| pyruvate dehydrogenase E1 beta subunit isoform 3 | gi|162458813 | N49 | Increase | 1.20:1 | 2.92:1 |
| vacuolar ATP synthase catalytic subunit A(V-ATPase A subunit) | gi|195658441 | N50 | Increase | 2.04:1 | 11.1:1 |
| 6-phosphogluconolctonase | gi|226493090 | N58 | Decrease | 6.78:1 | 1:1.14 |
| ATP synthase CF1 alpha subunit | gi|260677417 | N62 | Decrease | 1:1.97 | 1:3.01 |
| ATPase alpha subunit from chromosome 10 chloroplast | gi|19920165 | N63 | Decrease | 2.96:1 | 1:1.28 |
| ATP synthase CF1 alpha subunit | gi|50812525 | N67 | Decrease | 1.27:1 | 1:4.11 |
| Protein fate | |||||
| peptidylprolyl cis-trans isomerase | gi|226531796 | N40 | Qi319-96 Only | 1:3.50 | 0:1 |
| hsp70 | gi|308081377 | N53 | Increase | 4.02:1 | 3.06:1 |
| cpn60 chaperonin family protein | gi|242090109 | N56 | Increase | 1.69:1 | 4.71:1 |
| chaperonin | gi|195623400 | N72 | Decrease | 1.22:1 | 1.59:1 |
| Protein synthesis | |||||
| elongation factor G | gi|242076604 | N52 | Increase | 1.21:1 | 1:11.66 |
| Transcription/cellularcomm-unication/signal transduction | |||||
| GTP binding protein | gi|242061356 | N24 | Increase | 4.54:1 | 1:5.16 |
| translation initiation factor | gi|162462542 | N42 | Increase | 1:0 | 1.96:1 |
| RNA binding protein | gi|212722236 | N59 | Decrease | 1.97:1 | 2.81:1 |
| Cell rescue, defense and virulence | |||||
| CBS domain protein | gi|226490863 | N13 | Increase | 1:2.68 | 2.58:1 |
| peptide methionine sulfoxide reductase | gi|293332177 | N22 | Increase | 2.75:1 | 81.14:1 |
| peptide methionine sulfoxide reductase | gi|226532399 | N25 | Increase | 1.09:1 | 10.74:1 |
| hydroxyproline-rich glycoprotein family protein | gi|226507242 | N57 | Decrease | 1.19:1 | 1.89:1 |
| Unknown | |||||
| predicted protein | gi|224157625 | N60 | Decrease | 62.39:1 | 18.20:1 |
| unknown protein | gi|18419782 | N61 | Qi319 only | 2.00:1 | 1:0 |
| hypothetical protein | gi|218185826 | N65 | Decrease | 1:1.78 | 2.97:1 |
| hypothetical protein VOLCADRAFT_104026 | gi|302834273 | N66 | Decrease | 1:3.02 | 31.68:1 |
| clamp (CC)-tetratricopeptide repeat (TPR) proteins | gi|115446205 | N68 | Decrease | 3.84:1 | 1:7.29 |
| Secondary metabolism | |||||
| Caffeic acid O-methyltransferase | gi|33641714 | N30 | Increase | 1:3.35 | 1:1.86 |
| 3-N-debenzoyl-2-deoxytaxol N-benzoyltransferase | gi|226500072 | N31 | Increase | 1.81:1 | 1:2.71 |
| dehydroquinate dehydratase | gi|255070969 | N46 | Increase | 1.52:1 | 1:81.95 |
| ornithine carbamoyltransferase | gi|255538702 | N64 | Decrease | 84.38:1 | 30.71:1 |
| ketol-acid reductoisomerase | gi|212722020 | N70 | Decrease | 4.35:1 | 1:9.89 |
a: Name of protein identified by MALDI-TOF MS
b: Database accession numbers from NCBInr
c: Assigned spot number, as indicated in Fig. 2
d: “Increase” indicates significance at P < 0.05 and an increase in amount of at least 1.5-fold on the Qi319-96 gel under + P or –P conditions; “decrease”indicates significance at P < 0.05 and a decrease in amount of at least 1.5-fold on the Qi319-96 gel under + P or –P conditions compared withQi319
e: Specificity indicates the ratio of accumulation of a particular protein in leavesbetween Qi319-96 + P and Qi319-96 –P
f: Specificity indicates the ratio of accumulation of a particular protein in leavesbetween Qi319 + P and Qi319 – P