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. 2016 May 31;11:2463–2469. doi: 10.2147/IJN.S101881

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© 2016 Baert et al. This work is published and licensed by Dove Medical Press Limited

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FedF was efficiently incorporated inside GPs.

Notes: (A) The loading efficiency of FedF inside GPs was determined by BCA. Therefore, the unbound FedF protein in the wash fractions was measured by BCA against a BSA standard (0.05–1 mg/mL). (B) FedF incorporation inside the GPs was analyzed by Western blotting after digesting the particles with β-glucanases. FedF was detected with specific monoclonal antibodies against FedF. Lane 1 illustrates FedF, while the FedF released from the GPs upon digestion is shown in lane 2.

Abbreviations: GPs, β-glucan microparticles; BCA, bicinchoninic acid assay; BSA, bovine serum albumin.