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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2016 Feb 2;93:145–154. doi: 10.1016/j.freeradbiomed.2016.02.003

Figure 5. Impact of NO on protein and RNA degradation in stationary phase.

Figure 5

A. Cells carrying an IPTG-inducible, ssrA-tagged GFP were grown in the presence of inducer in both overnight and following cultures. At t=4 h, the inducer was removed, and the cultures were treated with 3 mM DPTA or left untreated. GFP levels were measured with a plate reader (Methods). Note that GFP in untreated cultures at t=8 h was not detectable. B–C. Total RNA were isolated from early stationary phase, DPTA-treated, and untreated late stationary phase cultures, and analyzed with a bioanalyzer. RNA integrity values range from 10 (intact) to 1 (totally degraded). For control groups (untreated), solvent was added to cultures. * indicates a statistical difference between untreated and DPTA treated groups (P-value<0.05, t-test). At least three biological replicates were performed for each experimental condition. Each data point represents the mean value ± standard error.