Table 2.
Mutations identified by re-sequencing
| Gene | Locus tag | ORF nt | DNA mutation | Coding change | Occurrence in strain |
|---|---|---|---|---|---|
| adhE | Tsac_416 | 1630 | G → A | G → D | M0734, M0863 |
| adhE | Tsac_416 | 1804 | C → T | S → L | M0699 |
| adhE | Tsac_416 | 1808 | A → G | E → G | M0694, M0700 |
| hfsA | Tsac_1550 | 159 | A → - | Frameshift | M0700 |
| hfsB | Tsac_1551 | 434 | A → - | Frameshift | M0734, M0863 |
| hfsB | Tsac_1551 | 652 | A → - | Frameshift | M0731 |
| hfsC | Tsac_1552 | 255 | A → - | Frameshift | M0699 |
| hfsD | Tsac_1553 | 193 | G → A | A → T | M0699 |
| hfsD | Tsac_1553 | 321 | A → T | R → S | M0863 |
| hfsD | Tsac_1553 | 787 | G → A | E → K | M0734 |