Fig. 8.

Unprocessed forms of PfPSD proenzyme do not interfere with processing or catalysis reactions of other variants.

A. Extracts were prepared from yeast psd1Δpsd2Δdpl1Δ strains expressing Mock, full-length PfPSD or PfPSDΔ70. The extracts as indicated in the figure were mixed and pre-incubated for 10 min prior start of the enzyme assay for decarboxylase activity. Data are means ± standard deviation for two experiments each performed in duplicate.

B. Cell free extracts of the psd1Δpsd2Δdpl1Δ mutant strain expressing Mock, PfPSDΔ50 or PfPSDΔ70 were mixed for 0 or 2 h as indicated. Western blot analysis was performed to detect the proenzyme and the mature β-subunit of PfPSDΔ70 and PfPSDΔ50 using anti-PfPSD.