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. Author manuscript; available in PMC: 2016 Jun 8.
Published in final edited form as: Traffic. 2015 Apr 20;16(5):534–554. doi: 10.1111/tra.12267

Figure 6. Transient co-expression of CaARA7 with organelle markers in epidermal cells of control untreated tobacco leaves and in epidermal cells of leaves infiltrated with wortmannin (WM).

Figure 6

A–D) In untreated cells the fluorescence of GFP-CaARA7 labelled organelles (A) frequently overlaps with the fluorescence of mCherry-AtARA7-labelled particles (B). Arrow heads in the merged image (C) indicate colocalizations; (D) is the corresponding scatter plot. E–H) Colocalization of mCherry-CaARA7 with CaARA6-GFP in control untreated epidermal cells of tobacco leaves (arrow heads in the merged image (G); (H) is the corresponding scatter plot). I–L) WM compartments (arrows) carry both mCherry-CaARA7 (I) and CaARA6-GFP (J). K) Is the merged image. Note characteristic WM compartments in the corresponding DIC image (L). M and N) Co-expression of wild-type GFP-CaARA7 (green) with the Golgi marker CD3-968-mCherry (red) (M, merged image) and cumulative scatterplot (N) show no colocalization. O and P) Co-expression of wild type green fluorescent GFP-CaARA7 with the red fluorescent TGN marker SYP61-mRFP (O, merged image) and cumulative scatterplot (P) illustrate lack of colocalization. Bars are 10 μm.