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. Author manuscript; available in PMC: 2016 Jun 8.
Published in final edited form as: Biochemistry. 2016 Mar 24;55(13):2043–2053. doi: 10.1021/acs.biochem.5b01282

Figure 2.

Figure 2

A. Primary sequence alignment of the Rev1-interacting regions (RIR) from human PolD3 subunit of Polδ and Polζ, Y-family TLS DNA polymerases Polη, Polι and Polκ, and a single-strand break and base excision repair protein, XRCC173. B. Overlay of 1H-15N HSQC spectra of the free 15N/13C Rev1-CT domain (blue) and its complex with the unlabeled PolD3 RIR-motif (magenta). B, C. Surface plasmon resonance (SPR) sensorgrams for the Rev1-CT domain injected over the PolD3-RIR (plot C) and Polκ-RIR (plot D) peptides immobilized on a sensor chip surface (top panels) and best fits of the steady state response values to a two-state binding model (bottom panels).