Table 2. PCR results with articular fluids infected with S. aureus.

Patient	Bacterial Load CFU/ml	Without capturea Ct spab	Vol. used after FcMBL capture	Ct spa after FcMBL capture
A	1.107	Invalid	100 μl	21
B	8.104	22	100 μl	24
C	1.104	30	100 μl	31
D	1.104	Invalid	100 μl	32
E	1.103	34	1 ml	31
F	2.102	37	1 ml	31
G1	1.102	Negative	100 μl	Negative
G2	1.102	Negative	5 ml	32
H	3.101	Negative	1 ml	34

^a Without capture, the volume defined for PCR is 100 μl as per manufacturer’s instructions.

^b Number of amplification cycles required to detect staphylococcal protein A (spa) gene as reported on the Genexpert software.