Fig 2. Correlation between CD24% and cisplatin sensitivity.

A) Tabulation showing that CD24+ is an indicator of poor outcome in multiple cancers. B) Tabulation showing that the positive or negative expression of CD24 is the discerning factor in isolating cancer stem cells/ tumor propagating cells in various cancers. C) Flow cytometry analysis of membrane bound CD24 and CD44 of the three laryngeal carcinoma lines show an increasing fraction of CD24% and D) Graphical representation of CD24% with R² = 0.804 showing a positive linear correlation with increasing cisplatin resistance in the three laryngeal carcinoma lines. E) Primary and secondary orospheres formed by the CD24+ (green) and CD24– (red) fractions. F) Graph of the number of orospheres formed as shown in E) with standard error of mean (t-test, n = 3). G) CD24+ residual fraction increase in proportion as it remains viable even after an IC-50 dose of cisplatin treatment. H) Graph with standard error of mean (t-test, n = 3) of the percent increase in proportion of the residual viable CD24+ fraction after an IC-50 dose of cisplatin treatment.