Fig 5. Enhanced cytotoxicity of the combination between terpinen-4-ol and biological treatment tools.

1x10⁴ CR DLD1 and HCT116 cells (A, B, E), and prostate CL-1 (C, D) cells were seeded in 96-well plates in complete medium. On the next day, terpinen-4-ol at a concentration of 0.01% and the humanized anti-CD24 antibodies (150μg/ml) (A, C), and terpinen-4-ol at a concentration of 0.01% and cetuximab at a concentration of 1μM (B,D,E) were added for 72 h. Cell survival was evaluated by the enzymatic MTT assay. Each bar represents the mean±SD of a set of data determined in triplicates. (F) 0.4X10⁶ HCT116 cells were seeded in 6-well plates in triplicates. The next day, co-transfection with PY4-SV40-LUC vector and pRL-CMV was performed. 24h after the transfection medium was replaced and the cells were exposed to cetuximab, different concentration of terpinen-4-ol or left untreated. Luc assay was performed 48 h post after the treatment. Each bar represents the mean±SD of a set of data determined in triplicates. *P < .05, **P < .05