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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Nature. 2016 Apr 6;532(7599):334–339. doi: 10.1038/nature17629

Figure 2. Antidepressant binding and recognition.

Figure 2

a, Graph of [3H](R/S)-citalopram saturation binding to wild-type (black, circles), ts2 (blue, squares), and ts3 (red, triangles) transporters, showing the average of two independent experiments, each performed in triplicate (error bars represent s.e.m.). b, Plot of a [3H]-paroxetine saturation binding from a representative experiment (error bars represent s.e.m. from triplicate measurements). c, Fo-Fc omit (S)-citalopram electron density (blue mesh), contoured at 3σ. The approximate positions of subsites A, B, C are shown. d, Anomalous difference electron density (green mesh), derived from Br-citalopram (yellow sticks) bound to the central site is shown (8.0σ contour level). e, Fo-Fc omit electron density for paroxetine, contoured at 3σ. f, Interactions of (S)-citalopram (dark green) in the central binding site. g, Interactions of paroxetine (pink) with residues in the central binding site.