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. 2016 Jun 8;11(6):e0156772. doi: 10.1371/journal.pone.0156772

Fig 3. Endogenous moR-21 downregulates Txndc5 expression.

Fig 3

(a) Txndc5 protein abundance in scrambled or moR-21 mimetic and scrambled or anti-moR-21 treated cells. Representative western blots are presented. Densitometry analysis for western blots is shown in the lower panel. Data are from at least 4 independent experiments and presented as fold change, compared with scrambled mimetic and scrambled anti-moR treated cells. (b) Upper panel: the moR-21 sponge was designed with bulged binding sites to avoid endonucleolytic cleavage by Ago2. Lower panel: the moR-21 sponge was constructed by inserting 7 copies of bulged moR-21 binding sites into the 3’UTR of a destabilized GFP reporter gene. (c) qRT-PCR analysis of Txndc5 mRNA in control or moR-21 sponge transfected HEK293 cells. Gapdh was used as an internal control for RT-qPCR. (d) immunoblot analysis of Txndc5 protein abundance in control or moR-21 sponge transfected HEK293 cells. Data are from 4 independent experiments. Values are mean ± SEM. The significance of differences between different treatments was determined by Student’s t-test. NS: non-significant, *: P<0.05, **: P<0.01, ***: P<0.001.