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. 2016 Jun 8;11(6):e0157186. doi: 10.1371/journal.pone.0157186

Fig 5. Mevalonate replacement reversed the inhibitory effects of simvastatin on BA-E cells.

Fig 5

BA-E cells were pretreated with 2 μM mevalonate and 25 μM simvastatin for 3 h before the stimulation of 10 ng/ml IL-5 for 24 h. (A) Chemotaxis assay using eotaxin as a chemokine was performed as described. (B) CCR3 mRNA expression was analyzed by RT/real-time PCR. Data were expressed as the combined mean ± SEM of n = 4 independent experiments. *P < 0.05, Mann–Whitney U test. (C) CCR3 protein expression (green color) on the surface of BA-E cells was revealed by immunocytochemical stain in different treatments.